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University of Washington RESEARCH SCIENTIST/ENGINEER 2 in Seattle, Washington

Req #: 230829


Posting Date: 02/05/2024

Closing Info: Open Until Filled

Salary: $4,800 - $6,800 per month

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. ( )

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.

The Immunology Department at the University of Washington School of Medicine has an outstanding opportunity for a Research Scientist/Engineer 2 (NE S UAW RSE) in the Gale laboratory. Research in the Gale laboratory is focused on understanding innate immune programming in response to RNA virus infection. We are using systems biology to define innate immune interactions of acute viral infection and correlates of vaccine-induced protection in preclinical efficacy studies.

The highly motivated candidate will perform standardized high-throughput RNA sequencing assays and have a strong commitment to the overall program and project goals.

  • Participate in sequencing group meetings to provide project status reports and present experimental results (10%).

  • Expectation to train group members on sequencing assay (5%).

  • Coordinate on sample acquisition/delivery and management; organize and maintain detailed records related to sample tracking and sequencing (5%).

  • Handling of human and nonhuman primate whole blood, tissues and single cell suspensions in an enhanced biosafety level 2 facility (BSL-2+) (10%).

  • Process culture and tissue-derived cell samples for single-cell RNA sequencing. Knowledge of 10X single cell RNA sequencing protocol a plus (5%).

  • Process culture and tissue samples for RNA and DNA isolation (10%).

  • Preparation of sequencing libraries for bulk, viral amplicon, pan-viral and single-cell RNA sequencing and associated tasks related to library QC and pooling (25%).

  • Ability to load prepared libraries directly onto sevquencer with preferred knowledge of Illumina NextSeq500 system (5%).

  • Operation and knowledge of Biomek i7 Hybrid Automated Workstation robotic liquid handler used to facilitate high-throughput sample processing for functional genomics analyses, including RNA isolation and rapid RNA sequencing library construction (20%).

  • Optimize and implement new sequencing assay pipelines that includes CITE-seq, ATAC-seq, and viral sequencing assay pipelines, and other assays as needed (5%). MINIMUM REQUIREMENTS

  • Bachelor’s degree in a related field and two years of relevant experience, or equivalent combination of education/experience.

  • Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. DESIRED QUALIFICATIONS

  • Bachelor’s degree in biological sciences or related field with 5 or more years of relevant post-graduate laboratory experience.

  • Relevant industry experience a plus.

  • General knowledge of methods in molecular biology and general understanding of principles behind viral infection and disease.

  • Experience performing wet bench lab protocols, including usage of pipettes, aliquoting and working on a 96-well plate format.

  • Experience with Microsoft Office Excel for data reporting and experimental calculations.

  • Must achieve deadlines and specified project milestones in an efficient manner.

  • Willingness to quickly change direction and priorities based on a rapidly evolving research program. Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.