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University of Washington RESEARCH SCIENTIST/ENGINEER 2 in Seattle, Washington

Req #: 215641

Department: DEPARTMENT OF LABORATORY MEDICINE AND PATHOLOGY

Posting Date: 11/22/2022

Closing Info: Open Until Filled

Salary: Salary and benefits are competitive. Salary is commensurate with qualifications and experience.

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For detailed information on Benefits for this position, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academi-staff-20210208.pdf)

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. The Heart Regeneration Program, a translational program within UW Medicine, is developing human pluripotent stem cell-derived cardiomyocytes as a cell therapeutic. The program has an immediate opening for a Research Scientist Engineer 2. Under the general guidance of Senior Scientists, this position will be required to work independently to reach established milestones and goals. This position will also be expected to work collaboratively with fellow research personnel, and redirect efforts to achieve task/project objectives or enhance the quality of the project when necessary. RESPONSIBILITIES: The typical job functions for this position will include: • Cell culture responsibilities: o Aseptic and sterile work techniques o Expansion, maintenance, and banking of undifferentiated human pluripotent stem cells (hPSCs) o Performing quality control assays for undifferentiated cell banks (karyotyping, mycoplasma testing, etc.) o Clonal isolation, expansion and banking of edited cell lines o Differentiation of human pluripotent stem cells towards various cardiovascular lineages (i.e. cardiomyocytes, epicardial cells, endothelial cells, smooth muscle cells) o Large scale banking of cardiomyocytes derived from hPSCs o Transfection of cultured cells • Gene editing; • Perform molecular analyses such as RT-PCR, western blotting, immunocytochemistry, and flow cytometry; • Assist with laboratory operations including ordering, regulatory compliance, and other key support as needed; • Prepare and maintain accurate protocols; • General lab maintenance; • Attend meetings as required; • Other duties as assigned MINIMUM REQUIREMENTS: • Bachelor’s degree in a life science related field • At least 2 years of experience in cell culture and molecular skills Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. ADDITIONAL REQUIREMENTS: • Excellent organizational and communication skills • Excellent writing skills • Ability to teach • Command of Microsoft Word, Excel, PowerPoint programs • Ability to work independently and as part of a diverse team DESIRED: • Hands-on experience with human ESC or iPSC culture and differentiation; • Experience with suspension bioreactors for cell culture • M.S. in cell and developmental biology, physiology, biochemistry, or biomedical engineering. Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.

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