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University of Washington RESEARCH SCIENTIST/ENGINEER 2 (GIACANI'S LAB) (Prostaff-Salaried/Temporary) in Seattle, Washington

Req #: 219501


Appointing Department Web Address:

Posting Date: 03/29/2023

Closing Info: Open Until Filled

Salary: $4,470-$6,573 per month

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.

The Department of Medicine’s Division of Allergy and Infectious Diseases (AID) has an outstanding opportunity open for a Temporary Research Scientist/Engineer 2. Position Purpose:

The Research Scientist/Engineer 2 will perform laboratory research work in support of studies on syphilis vaccine discovery and syphilis/T. pallidum pathogenesis using a variety of immunological/molecular techniques and the rabbit model for syphilis. The overall goal of the research is to find and validate new vaccine candidates for syphilis, and understanding the molecular mechanisms being the syphilis's abilities to cause infection. This position's funding is currently for 6 months with possible extension up to 12 months.

Duties and Responsibilities:

Under minimal supervision, duties include (but are not limited to): • Prokaryotic and eukaryotic cell culture; expression and purification of recombinant proteins, perform rabbit procedures (e.g. immunization, infection, blood withdrawal) as per animal protocol immunizations and monitoring after infections. (30%) • Molecular analysis of samples using qPCR, DNA and RNA extraction, reverse transcription. (20%) • Help with the training of undergraduate students joining the lab. (20%) • Help with the management of chemical inventories, Help ensuring that the lab is in compliance with EHS standards. (20%) • Maintain lab supplies and very detailed lab notebooks. (10%)

Minimum Qualifications:

  • Bachelor's degree in Biology, Biochemistry, Microbiology, Immunology, or related field and or with at least 2 years of experience in: • Molecular biology techniques such as DNA isolation/manipulation, PCR, sequencing, electrophoresis. Western blot, ELISA, cloning, expression of recombinant proteins • Good sterile technique and and the ability to perform tissue culture techniques and maintain cultures. • Willingness to work with animal models (rabbits) and BSL2 organisms. • Ability to prepare reagents, manage data and keep a very detailed notebook. • A high level of motivation, good organizational skills and the ability to work as a member of a team.

Experience has substitute for educational requirement.

Desired Qualifications:

• Previous work with Treponema pallidum • Previous work with laboratory rabbits.

Application Process:

The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter, and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.