Work in Washington Veterans Jobs

Req #: 235913

Department: DEPARTMENT OF MEDICINE: ALLERGY & ID

Posting Date: 07/12/2024

Closing Info: Open Until Filled

Salary: $3,887 - $5,000 per month

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230119_a11y.pdf)

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.

Department of Medicine: Allergy and Infectious Diseases has an outstanding opportunity for Research Scientist/Engineer 1. Field of research this position is engaged in:
The staff member will be a part of the Structural genomics center (SSGCID) in the Department of Medicine, Division of Allergy & Infectious Disease, CERID located aat the UW South Lake Union Campus.

Purpose of the research project(s) this position supports:
This position will assist in the structural genomics pipeline to clone, express, purify, and assay bacterial and parasite proteins for x-ray crystallography, biochemical assays, ELISA and structure-based drug design.

RESPONSIBILITIES:

Assist in the cloning, expression testing and the High throughput upscale of SSGCID targets. Produce 2 L cultures of 8-16 unique proteins per week from targets which have been nominated by the research community. This includes researching each target for additives and buffers to enhance the chance of successful purifications. (55%)

Data analysis, SharePoint updates and preparation of reports. (15%)

Development and performance thermal melt assays using the proteins produced within SSGCID to enzymatically test research compounds for effectiveness. (10%)

Supervise lab assistants in pipeline procedures. (10%)

Development of ELISA using the protein produced to screen human sera for detection of infectious diseases. Screening of target's protein expression and analysis of SDS page gels to determine if upscale was successful. (5%)

Making all necessary reagents and media to perform experiments. (5%)

Research Sponsors/Stakeholders: Team member of the UW unit of the NIH contract sponsoring the ongoing efforts of SSGCID, the Seattle Structural Genomics Center for Infectious Diseases. SSGCID is one of two Centers funded by NIAID in response to RFP-NIH-NIAID-DMID-07-19, RFP-NIAID-DMID-NIHAI2011124 and RFP-NIAID-DMID-NIHAI2016061. It is a consortium of five institutions (SCRI, UW SLU, UW IPD, Pacific Northwest National Laboratory, and the University of Kansas). SSGCID’s primary mission is to determine the structure of ca. 70 protein targets from NIAID Category A-C agents, as well as emerging and re-emerging infectious disease organisms, each year for a period of five years. SSGCID clones and proteins are available free of charge to the larger scientific community.

MINIMUM REQUIREMENTS:

Bachelor's Degree in Biology or Chemistry or a science related field and one year of relevant experience, OR Equivalent combination of education/experience.

Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. ADDITIONAL REQUIREMENTS:

• Ability to perform molecular biology techniques such as DNA isolation/manipulation, PCR, sequencing, and electrophoresis. • Ability to produce upscale cultures of recombinant proteins and purified proteins through chromatography methods - (Size exclusion and ion exchange chromatography) for produce of purified protein for use in crystallography trials. • Ability to develop and perform ELISAs on human serum samples. Good sterile technique and the ability to maintain bacterial cultures is needed. • Ability to prepare reagents, manage data and keep a detailed notebook. • A high level of motivation, ability to research, ability to troubleshoot, good organizational skills and the ability to work as a member of a team. • Able to train and supervise student employees.

DESIRED:

• High throughput cloning methods of bacterial and viral targets. • Protein expression and purification techniques including the use of sonication, size exclusion and ion exchange chromatography. • High Throughput methods developing assays in a 96-well format and data analysis of results. • Familiarity with database software (SQL, Access, or Sharepoint). • Knowledge of Aktaexplorrer and NGC chromatography instruments is a plus. • Use of baculovirus expression techniques. • Some knowledge of bioinformatics

Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.