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University of Washington RESEARCH SCIENTIST in Seattle, Washington

Req #: 231543

Department: DEPARTMENT OF MEDICINE: NEPHROLOGY

Posting Date: 03/05/2024

Closing Info: Open Until Filled

Salary: $5,427 - $11,051 per month

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academic-staff-20230701_a11y.pdf )

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world.

UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty.

The University of Washington (UW) is proud to be one of the nation’s premier educational and research institutions. Our people are the most important asset in our pursuit of achieving excellence in education, research, and community service.

The Freedman Lab in the Division of Nephrology, Department of Medicine, is seeking a Research Scientist (Research Scientist/Engineer 3 (E S UAW RSE)) to lead projects using human pluripotent stem cells to develop new treatments for kidney disease, under the general guidance/supervision of Associate Professor Benjamin Freedman. The Research Scientist will combine stem cell culture and differentiation with bioengineering, microfluidics, and pre-clinical animal models to build next-generation models of human kidney regeneration and disease. This position will be responsible for contributing to preparation of reports, papers, presentations and new proposals. He/she will contribute to positive colleague and investigator relationships through interactions on current grants. The successful applicant will also assist Dr. Freedman with mentoring of trainees. Experience with animals is a plus.

This position will be located at South Lake Union campus of UW Medicine, a bastion of stem cell research in the innovative center of Seattle. The successful candidate will be detail-oriented, reliable, highly organized, possess a high degree of accountability for quality of work performed, can work independently and has excellent technical skills.

DUTIES AND RESPONSIBILITIES Experimental duties include: 60%

  • Animal work – husbandry, injection, analysis and surgery relevant to kidneys

  • Cell culture – daily differentiation and maintenance of human pluripotent stem cells

  • Microscopy – immunofluorescence, confocal, wide-field, live cell

  • Microfluidics – bioengineering, device preparation, input/output bioanalysis

  • Genome editing – guide RNA construction, transfection, screening

  • Drug discovery – design and execution of high throughput screening assays

  • Biochemistry and molecular biology – qPCR, electrophoresis, immunoblotting, cloning

  • Computing and analysis – Illustrator, Powerpoint, Excel, Word, ImageJ, HTML

  • Documentation – daily record of work performed in files and laboratory notebookLab duties: 20%

  • Mentor research trainees and undergraduates; contribute to improvements in efficiency

  • Orient/train new staff

  • Manage projects of moderate size and scope

  • Assemble and organize research teams

  • Lead projects or project tasks toward research objectives

  • Inventory management for reagents/orders/cells; track expenses

  • Ensure compliance with lab regulations and completion of corresponding paperworkScholarship: 10%

  • Contribute data and ideas for new projects

  • Drafting of new grants with minimal supervision

  • Preparation of primary research manuscripts with minimal supervision

  • Writing invited manuscripts

  • Assist in seeking/acquiring additional funding

  • Manage relationships with grants sponsors and progress reportsAnimal care includes: 10%

  • Data collection/maintenance management for rodent colony

  • Updating and ensuring compliance with animal welfare protocols

  • Oversight of junior trainees working in the animal facility

  • Demonstration and training of surgeries

  • Developing new animal protocols MINIMUM REQUIREMENTS

  • Bachelor’s degree in biology, bioengineering, biophysics or related field and at least four years of experience or equivalent combination of education and experience.

  • Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. ADDITIONAL REQUIREMENTS

  • Cell culture skills and experience; strong computer skills including Excel, statistical and/or graphical software (ex. Adobe Illustrator, ImageJ, Powerpoint); excellent organizational and communication skills; ability to manage diverse tasks; flexibility in work hours; a thirst for scientific knowledge and progress; comfort working with animal model; and a high degree of integrity. DESIRED QUALIFICATIONS

  • Experience with human pluripotent stem cells, high throughput screening, genome editing (CRISPR/Cas9), or rodent microsurgery. Application Process: The application process may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are access ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.

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