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University of Washington RESEARCH SCIENTIST 1 in Seattle, Washington

Req #: 189697

Department: DEPARTMENT OF PHARMACOLOGY

Posting Date: 05/03/2021

Closing Info: Open Until Filled

Salary: Salary is commensurate with qualifications and experience.

Shift: First Shift

Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academi-staff-20210208.pdf)

As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem-solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. The Department of Pharmacology has an excellent opportunity for a Research Scientist 1. Research in the Scott lab focuses on studies of spatial and temporal specificity of signal transduction events that are controlled by anchoring proteins or AKAPs. These molecules facilitate rapid signal transduction by optimally positioning protein kinases and phosphatases in the vicinity of their activating signals and close to their substrates. The Research Scientist/Engineer 1 will perform research focusing on the processes involved in signal transduction events which are regulated by a family of A-Kinase Anchoring Proteins (AKAPs) and how these intracellular communication networks can be used to regulate key physiological processes such as neurotransmission, heart rate, glucose dependent homeostasis and cell cycle. The role of AKAPs in disease models of liver cancer, breast cancer and Cushing’s Syndrome will be researched. RESPONSIBILITIES • Maintenance of a mouse colony comprising of multiple fragile strains. • Perform mouse genotyping and sterile tissue culture for designated projects. • Protein expression and purification. • Culture of human and mouse cancer cell lines and prepare cells for analysis using approaches including PCR, gene cloning and immunohistochemistry. • Microscopy experiments using both wide-field and super resolution imaging. • Prepare experimental reagents. • Document information and maintain detailed records and daily logs in a laboratory notebook. • Data analysis. • Participate in group lab meetings and other academic activities by performing regular presentations of data to the research group. • Maintain awareness of lab safety procedures and documentation of lab safety records. • Contribute results, text and images for grant submissions and presentations. • Present research results at laboratory, departmental and national meetings. • Complete special projects as necessary by assisting senior research scientists. • Other duties as assigned. MINIMUM REQUIREMENTS • Bachelor's degree in Biology, Biochemistry, Pharmacology or related field of science. • At least three months of laboratory job-related experience, which can include experience gained as an undergraduate researcher. • Ability to work with mice. • Knowledge in molecular biology techniques. • Previous experience culturing cell lines, sterile technique and PCR. This can include experience gained as an undergraduate researcher. Equivalent education and/or experience may substitute for minimum requirements. DESIRED REQUIREMENTS • Attention to detail, ability to acquire new skills with minimal training, and experience with management of multiple mouse colonies. CONDITIONS OF EMPLOYMENT The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment, you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed.

University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.

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